A Evaluation of Placentrex as a novel storage medium for avulsions: an invitro study
Downloads
ABSTRACT
Tooth avulsion is one of the common traumatic injury results in complete extrarticulation of tooth from its socket since resulting in damage to the periodontal tissues. Maintaining viability of the periodontal cells remains a challenge to the clinician to reimplant the avulsed tooth. Since various avulsion media are used in dentistry a novel medium Placentrex derived from 0.1 gm. of fresh term, sterilized, infection-free human placenta which has unique pharmacological effects like enhancement of wound-healing, anti-inflammatory, antioxidant and analgesic effect is compared with HBSS and Propolis media in freshly extracted, non carious, non restored, periodontal diseases free human teeth which are indicated for orthodontic treatment purposes were used. The extracted teeth were placed in the enzyme solution to facilitate detachment of the cells and after ten minutes teeth were removed and 1 ml of solution were collected through micropipette and centrifuged. The stained cells were counted in Neubauer’s chamber under a light microscope at 10× magnification. Conclusion: Placentrex maintained PDL cell viability almost similar to Propolis and significantly better than HBSS.
KEY WORDS : Avulsions, Avulsion media, HBSS, Reimplantation,
Downloads
Andreasen JO, Andreasen FM. Avulsions. In Andreasen JO, Andreasen FM (eds). Textbook and color atlas of traumatic injuries of the teeth, 3rd ed. pp 383-425. Copenhagen: Munksgard, 1994
Glendor U, Halling A, Andreasen L, Eilert-Petersson E. Incidence of traumatic tooth injuries in children and adolescents in the county of Vastmanland, Sweden. Swed Dent J 1996;20: 15-28
Fountain S B, Camp J H. Traumatic injuries. In: Cohen S, Burns R C, eds. Pathways of the pulp, 7th edn. St. Louis: C V Mosby, 1998: 585-90
Panzarini SR, Gulinelli JL, Poi WR, Sonoda CK, Pedrini D, Brandini DA. Treatment of root surface in delayed tooth replantation: a review of literature. Dent Traumatol 2008;24:277-82
Andreasen JO, Borum MK, Jaconsen HL, Andreasen FM. Replantation of 400 avulsed permanent incisors. Part 4. Factors related to periodontal healing. Endod Dent Traumatol 1995;11:76-89
Andreasen JO, Andreasen FM, Skeie A, Hjørting- Hansen E, Schwartz O. Effect of treatment delay upon pulp and periodontal healing of traumatic dental injuries – a review article. Dent Traumatol 2002;18:116-28
Soares AdeJ, Gomes BP, Zaia AA, Ferraz CC, de Souza-Filho FJ. Relationship between clinical-radiographic evaluation and outcome of teeth replantation. Dent Traumatol 2008; 24:183-88
Tonello G, Daglio M, Zaccarelli N, Sottofattori E, Mazzei M, Balbi A. Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent. J Pharm Biomed Anal 1996; 14:1555-60
.Biswas TK, Bhattacharya NP, Bhattacharya S, Mukherjee B. Wound healing activity of human placental extracts in rats. Acta Pharmacol Sin 2001; 22: 1113-16
Lee KH, Kim TH, Lee WC, Kim SH, Lee SY, Lee SM Anti-inflammatory and analgesic effects of human placenta extract. Nat Prod Res 2011; 25(11):1090-100
Martin MP, Pileggi R. A quantitative analysis of Propolis: a promising new storage media for avulsion. Dent Traumatol 2004; 20:85-89
Gopikrishna V, Baweja PS, Venkateshbabu N, Thomas T, Kandaswamy D. Comparison of coconut water, propolis, HBSS and milk on PDL survival. J Endod 2008; 34:587-89
Priya Rajendran, Nettiyat OOmmen Varghese, Jolly Mary Varughese, Elingo Murugaian. Evaluation, using extracted human teeth, of Ricetral as a storage medium for avulsions – an invitro study. Dent Trautmatol 2011; 27:217-20
Polverini PJ, Leibovich SJ. Induction of neovascularization invivo and endothelial proliferation invitro by tumor associated macrophages. Lab Invest 1984; 51:635-42
Andreasen JO. Exarticulation in “traumatic injuries of the teeth”. 2nd ed. Copenhagen, Denmark: Mungsgaard, 1981
Andreasen JO. Effect of extra-alveolar period and storage media upon periodontal and pulpal healing after replantation of mature permanent incisors in monkeys. Int J Oral Surg 1981;10:43-53
Blomlof L, Otteskog P. Viability of human periodontal ligament cells after storage in milk or saliva. Scand. J. Dent. Res 1980; 88:436-40
Huang SC, Remeikis NA, Daniel JC. Effects of long-term exposure of human periodontal ligament cells to milk and other solutions. J Endod 1996; 22 (1): 31-33
Andreasen JO, Kristerson L. The Effect of Limited Drying or Removal of the Periodontal Ligament. Acta Odontologica Scandinavica 1981; 39(1):1-13
Sigalas E, Regan JD, Kramer PR, Witherspoon DE, Opperman LA. Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth. Dent tramautal 2004; 20:21-28
Hiltz J, Trope M. Vitality of human lip fibroblasts in milk, Hanks balanced Salt solution and Viaspan storage media. Dent Trautmatol 1991; 7:69-72
Krasner P, Person P. Preserving avulsed teeth for replantation. J Am Dent Assoc 1992; 123:80-88
Viuda-Martos M, Ruiz-Navajas Y, Fernández-López J, Pérez-Alvarez JA. Functional properties of honey, propolis and royal jelly. J Food Sci 2008; 73:117-24
Kujungiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal, and antiviral activity of propolis of different geographical origins. J Ethnopharmacol 1999; 64:235-40
Park SY, Phark S, Lee M, Lim JY, Sul D. Anti-oxidative and anti-inflammatory activities of placental extracts in benzo[a]pyrene-exposed rats. Placenta. 2010; 31(10):873-79
Sur TK, Biswas TK, Ali, Mukherjee B. Anti-inflammatory and anti-platelet aggregation activity of human placental extract. Acta Pharmacol Sin 2003; 24(2):187-192
Chang CJ,Yen ML, Chen YC, Chien CC, Huang HI, Bai CH, Yena BL. Placenta-derived multipotent cells exhibit immunosuppressive properties that are enhanced in the presence of interferon γ. Stem Cells 2006; 24:2466–77
Nath S, Bhattacharyya D. Cell adhesion by aqueous extract of human placenta used as wound healer. Indian J Exp Biol 2007; 45:732-38
Chakraborty PD, Bhattacharya D. In vitro growth inhibition of microbes by human placental extract. Current science 2005; 88(5):782-86
Copyright (c) 2021 International Journal of Scientific Research and Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
