Co-Deposition of Serum Amyloid P Component in mouse Brain following Chronic Inflammatory Mice

Background: Prolonged injection of casein results in systemic amyloidosis in mice with impaired hepatic functions due to excessive serum amyloid P component (SAP) deposition in liver, spleen, kidney and brain. Methods: We have carried out radiolabeling, fluorescence immuno staining and brain endothelial immune staining studies to source the serum amyloid P component (SAP) that present in the brain of the affected mice. Results: The experiments suggest that the systemic amyloidosis results in selective crossing of SAP proteins through blood brain barrier (BBB). Injection of 125I SAP to casein treated mice resulted in the accumulation of this proteins in the liver to the maximum extent and to about 20% (compared to liver) localization in the brain. Immuno histochemical studies also indicate that the abundance of SAP in the mice brain following chronic inflammatory condition. Conclusion: The present work provides a basis for investigation on the effect of chronic inflammation to the