Isolation, Partial Purification and Characterization of a Cold Active Lipase from Pseudomonas sp., Isolated from Satopanth Glacier of Western Himalaya, India

A Psychrotrophic bacterium producing cold active lipase enzyme at low temperature was isolated from soil sample of Satopanth glacier. It was identified as Pseudomonas fluorescens based on colony morphology, biochemical characteristics. Out of the seven selected lipase producing isolates the best one SGPR-4 was showing activity maximum at 20oC temperature. The cold active lipase was purified to 6.44 fold by DEAE cellulose column chromatography. The bacterial strain SGPR-4 showed maximum activity at pH 8.0 and temperature 20°C and it showed thermostability up to 30oC. Therefore these properties of enzyme could be explored for industrial application and commercialization